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RAS BiologyЦитология Cell and Tissue Biology

  • ISSN (Print) 0041-3771
  • ISSN (Online) 3034-6061

Design and selection of guides for CRISPR/Cas9-mediated knockout of the Kcnv2 gene in mouse cells

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PII
10.31857/S0041377124050036-1
DOI
10.31857/S0041377124050036
Publication type
Article
Status
Published
Authors
E. N. Antonova
  • Affiliation:
    Moscow Institute of Physics and Technology (National Research University)
    Lomonosov Moscow State University
    Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies
Volume/ Edition
Volume 66 / Issue number 5-6
Pages
420-437
Abstract
Mutations in the human KCNV2 gene cause a rare hereditary disease — cone dystrophy with supernormal rod response (CDSRR), characterized by progressive vision loss and impaired color discrimination. The KCNV2 gene encodes the Kv8.2 subunit of a potassium channel that is critical for the normal function of retinal photoreceptors. Gene therapy offers a promising treatment approach for this condition. To test the efficacy of gene therapy, an appropriate experimental disease model, such as a knockout mouse model, is required. This study focused on selecting optimal guide RNAs for knocking out the Kcnv2 gene using the CRISPR/Cas9 system and testing their efficiency in a mouse cell line. The selected guide RNAs can be utilized to generate a Kcnv2-/- mouse model.
Keywords
гены Kcnv2 KCNV2 гидовая РНК делеция Cas9
Date of publication
15.11.2024
Year of publication
2024
Number of purchasers
0
Views
11

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